Western blot analysis of Cas9 in HEK293 cell lysates. Samples were incubated in Cas9 monoclonal antibody (Product # MA5-47461). [1] protein standard (red), [2] non-transfected cells, and [3] transfected cells with GFP-Cas9 (C-terminal 803-1053 amino acids of S. aureus CAS9) fusion construct. Strong band at about 53 kDa corresponds to the GFP-CAS9 fusion protein.
Supplier Page from Thermo Fisher Scientific for Cas9 Antibody